DNA

Part:BBa_K5115034:Design

Designed by: Liyue Chen   Group: iGEM24_Fudan   (2024-09-19)


csoS operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
    Illegal NotI site found at 6599
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2805
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
    Illegal AgeI site found at 4933
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


Design Notes

Source

The part is originally identified in a species of cyanobacteria, Halothiobacillus neapolitanus. In our experiment, the CsoS2 part was obtained from a plasmid sourced from the Addgene repository (plasmid #140856).

References